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IR210-763 HER2 / ERBB2 antibody3
Home Products Primary antibody Cancer Marker IR210-763 HER2 / ERBB2 antibody
IR210-763

IR210-763 HER2 / ERBB2 antibody

Host : Rabbit
Clonality : Polyclonal
Reactivity : Human, Mouse, Rat
Package : 100 μl, 25 μl
Blocking : 3%BSA
MW (kDa): 180-185

Overview

Product Name

HER2 / ERBB2 antibody

Product Number

IR210-763

Gene Description

erb-b2 receptor tyrosine kinase 2

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human , mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:200 – 1:400

Immunohistochemistry (Paraffin) 1:200 – 1:250

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.14 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IR210-763 anti-HER2 / ERBB2 antibody WB image

All lanes : Anti-HER2 / ERBB2 antibody - at 1/500 dilution

 

Lysates/proteins at 30 μg per lane

This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with IR210-763 overnight at 4°C.  

 

  

Image

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IR210-763 anti-HER2 / ERBB2 antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling HER2 / ERBB2 with IR210-763 at 1/200.

Image

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IR210-763 anti-HER2 / ERBB2 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR210-763 anti- HER2 / ERBB2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Cancer Marker 1249675
Attachments:
IR210-763_HER2.pdf