|
Catalog |
Component |
Clonality |
Reactivity |
App |
|
EpCAM antibody Extracellular domain |
Rab-pAb |
H, M |
WB, IF, IHC, FC, CTC |
|
|
EpCAM antibody Intracellular domain |
Rab-pAb |
H, M, R |
WB, IF, IHC, CTC |
|
Catalog Number: IR218-3 Anti-EpCAM antibody (Extracellular domain) |
Package size: 25, 100 μl Store at: -20°C MW (kDa): 34-40 |
||
|
Overview |
|||
|
Product Name |
EpCAM antibody (Extracellular domain) |
||
|
Product Number |
|||
|
Gene Description |
epithelial cell adhesion molecule |
||
|
Clonality |
Polyclonal |
||
|
Host |
Rabbit |
||
|
Species Reactivity |
Human, Mouse |
||
|
Recommended Applications Dilutions |
Western Blot 1:1000 – 1:2000 Immunofluorescence 1:200 – 1:400 Immunohistochemistry (Paraffin) 1:100 – 1:150 Flow Cytometry 1:50 – 1:200 |
||
|
Storage Buffer |
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
||
|
Concentration |
0.54 mg/ml |
||
|
Purity |
Affinity column purified |
||
|
Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
||
|
Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
||
|
IR218-3 anti-EpCAM antibody WB image |
IR218-3 anti- EpCAM antibody IHC image |
||
|
|
|
||
|
Anti-EpCAM antibody - at 1/1000 dilution Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR218-3 overnight at 4°C. |
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with IR218-3 at 1/200 RT 1hr. |
||
|
IR218-3 anti- EpCAM antibody FACS image |
IR218-3 anti- EpCAM antibody ICC/IF image |
|
|
|
|
Product：EpCAM antibody Catalog：IR218-3 Cell : MCF-7 Data from : National Chiao-Tung University |
Cell : MCF-7 (DAPI : 1-300, Phalloidin : 1-300) EpCAM, (IR218-3) : 1-200 Anti-rabbit 594 : 1-500 NikonEpi-fluorescence (A-ken) 60x Data from : National Yang-Ming University |
|
IR218-3 anti-EpCAM antibody WB image |
IR218-3 anti- EpCAM antibody IHC image |
|
|
|
|
Anti-EpCAM antibody - at 1/1000 dilution Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR218-3 overnight at 4°C. |
Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with IR218-3 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0 Data from: Li-Tzung Biotechnology. Co |
