Overview

Product Name

Fibronectin antibody

Product Number

IR48-147

Gene Description

fibronectin 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:100 – 1:200

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.45 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR48-147 anti-Fibronectin antibody WB image

IR48-147 anti-Fibronectin antibody IHC image

All lanes : Anti-Fibronectin antibody at 1/500 dilution. Lysates at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3%BSA for an hour before being incubated with IR48-147 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Fibronectin with IR48-147 at 1/100.

Image

IR48-147 anti-Fibronectin antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR48-147 anti-Fibronectin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.