IR55-179 E-cadherin antibody (Extracellular domain)
Host :Rabbit
Clonality :Polyclonal
Reactivity :Human
Package : 100 μl, 25μl
Blocking : 3%BSA
MW (kDa): 130-135
Overview | ||
Product Name | E-cadherin antibody | |
Product Number | IR55-179 | |
Gene Description | cadherin 1, type 1 | |
Clonality | Polyclonal | |
Host | Rabbit | |
Species Reactivity | Human | |
Recommended Applications Dilutions | Western Blot 1:500 – 1:1000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:100 – 1:200 | |
Storage Buffer | 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative | |
Concentration | 0.22 mg/ml | |
Purity | Affinity column purified | |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C | |
Notes | Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. | |
Image | ||
IR55-179 anti-E cadherin antibody WB image | ||
All lanes : Anti-E cadherin antibody at 1/500 dilution
Lysates/proteins at 60 μg per lane This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with IR55-179 overnight at 4°C. | ||
Image | ||
IR55-179 anti-E cadherin antibody WB image | ||
All lanes : Anti-E cadherin antibody at 1/500 dilution
Lysates/proteins at 60 μg per lane This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with IR55-179 overnight at 4°C. | ||
Image | ||
IR55-179 anti-E cadherin antibody IHC image | ||
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-cadherin with IR55-179 at 1/100. | ||
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