Overview

Product Name

E-cadherin antibody

Product Number

IR55-179

Gene Description

cadherin 1, type 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:100 – 1:200

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.22 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

IR55-179 anti-E cadherin antibody WB image

All lanes : Anti-E cadherin antibody at 1/500 dilution

Lysates/proteins at 60 μg per lane

This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with IR55-179 overnight at 4°C.

Image

IR55-179 anti-E cadherin antibody WB image

All lanes : Anti-E cadherin antibody at 1/500 dilution

Lysates/proteins at 60 μg per lane

This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with IR55-179 overnight at 4°C.

Image

IR55-179 anti-E cadherin antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-cadherin with IR55-179 at 1/100.