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IR11-31 Tuj1 antibody
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IR11-31

IR11-31 Tuj1 antibody

Host :Rabbit
Clonality :Polyclonal
Reactivity :Human, Mouse, Rat
Package : 100 μl, 25μl
MW (kDa): 55

Overview

Product Name

Beta Tubulin 3/ Tuj1 antibody

Product Number

IR11-31

Gene Description

tubulin, beta 3 class III

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human , mouse, rat

Recommended

Applications Dilutions

Western Blot 1:5000 - 1:10000

Immunofluorescence 1:200 – 1:500

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.57 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

b2f75d06d4a2c25f33c4a988a903ff08.png

IR11-31 anti- Beta Tubulin 3/ Tuj1 antibody WB image

All lanes : Anti- Beta Tubulin 3/ Tuj1 antibody -at 1/5000 dilution

 

Lysates/proteins at 20 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR11-31 overnight at 4°C.

 

 

 

 

Image

358a429251f199326eeb7a1a80f5055e.png

IR11-31 anti- Beta Tubulin 3/ Tuj1 antibody WB image

All lanes : Anti- Beta Tubulin 3/ Tuj1 antibody -at 1/5000 dilution

 

Lysates/proteins at 20 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR11-31 overnight at 4°C.

Image

6c0046e5326879ec05997a2689651b84.png

IR11-31 anti- Beta Tubulin 3/ Tuj1 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR11-31 anti-Tuj1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Image

489796e531aea56294836010604f1fc6.png

IR11-31 anti- Beta Tubulin 3/ Tuj1 antibody ICC/IF image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Tuj1 with IR11-31 at 1/100.

IReal Biotechnology Co., Ltd.   

7F., No. 9, Sec. 3, Zhonghua Rd., North Dist., Hsinchu City 300, Taiwan (R.O.C.)

Website : www.irealbio.com        TEL : 03-526-0005       E-mail : irealbio@irealbio.com

Neuroscience 1237283
Attachments:
IR11-31_Tuj1.pdf