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GFAP ( Astrocyte Markers ) IR15-44 GFAP antibody 3
Home Products Primary antibody Neuroscience GFAP ( Astrocyte Markers ) IR15-44 GFAP antibody
IR15-44

GFAP ( Astrocyte Markers ) IR15-44 GFAP antibody

Host :Rabbit
Clonality :Polyclonal
Reactivity :Human, Mouse, Rat
Package : 100 μl, 25μl

Catalog

Component

Clonality

Reactivity

App

 IR15-44

GFAP antibody 

Rab-pAb

H, M, R

WB, IF, IHC



Overview

Product Name

GFAP antibody

Product Number

IR15-44

Gene Description

glial fibrillary acidic protein

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:1000-1:2000

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Frozen) 1:100 – 1:300

Immunohistochemistry (Paraffin) 1:100 – 1:300

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.31 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR15-44 anti-GFAP antibody WB image

IR15-44 anti-GFAP antibody IHC image

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All lanes : Anti-GFAP antibody at 1/1000 dilution

Lysates/proteins at 60 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose was then blocked for an hour before being incubated with IR15-44 overnight at 4°C.

 Immunohistochemical analysis of paraffin embedded Human brain tissue labeling GFAP antibody with IR15-44 at 1/100.

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IR15-44 anti-GFAP antibody IF image

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IR15-44 anti-GFAP antibody IHC image

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Immunofluorescent analysis.

Sample : primary cortical neurons

Red : GFAP (IR15-44) : 1-200

Blue : DAPI was used as the nuclear counter stain.

Fixed :  4% paraformaldehyde at RT for 20 min.

Immunohistochemical of frozen sections.

Sample : mouse cerebellum.

Green : GFAP (IR15-44) : 1-200

Anti-rabbit 488 : 1-500

IR15-44 anti-GFAP antibody ICC/IF image

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IR15-44 anti-GFAP antibody IHC image

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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR15-44 anti-GFAP antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical of frozen sections.

Sample : mouse cerebellum.

Green : GFAP (IR15-44) : 1-200

Anti-rabbit 488 : 1-500

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Attachments:
IR15-44_GFAP.pdf