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IR12-33 MAP2 antibody3
IR12-33

IR12-33 MAP2 antibody

Host :Rabbit
Clonality :Polyclonal
Reactivity :Human, Mouse, Rat
Package : 100 μl, 25μl

Overview

Product Name

MAP2 antibody

Product Number

IR12-33

Gene Description

microtubule-associated protein 2

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500 1:1000

Immunofluorescence 1:200 – 1:300

Immunohistochemistry (Paraffin) 1:100 – 1:150

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.35 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Customer feedback Image

IR12-33 anti-MAP2 antibody IF image

IR12-33 anti-MAP2 antibody IHC image

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Immunofluorescent analysis.

Sample : primary cortical neurons

MAP2 antibody (IR12-33) : 1-200

Anti-rabbit 488 : 1-500

Fixed :  4% paraformaldehyde at RT for 20 min.

Immunohistochemical analysis of paraffin embedded Human brain and mouse tissue labeling MAP2 antibody with IR12-33 at 1/100.

IR12-33 anti-MAP2 antibody ICC image

IR12-33 anti-MAP2 antibody WB image

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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR12-33 anti-MAP2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

All lanes : Anti-MAP2 antibody at 1/500 dilution

Lysates/proteins at 60 μg per lane

This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR12-33 overnight at 4°C.

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Attachments:
IR12-33_MAP2.pdf