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IR8-23 Nestin / NES antibody3
Home Products Primary antibody Neuroscience IR8-23 Nestin / NES antibody
IR8-23

IR8-23 Nestin / NES antibody

Host :Rabbit
Clonality :Polyclonal
Reactivity :Human, Mouse, Rat
Package : 100 μl, 25μl

Overview

Product Name

Nestin antibody

Product Number

IR8-23

Gene Description

nestin

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:100 – 1:200

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.35 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR8-23 anti-Nestin antibody WB image

IR8-23 anti-Nestin antibody WB image

IR8-23 anti-Nestin antibody IHC image

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Anti-Nestin antibody at 1/1000 dilution

Lysates at 30 μg per lane.

Sample : Neuron 2A

Nestin antibody (IR8-23) : 1-1000

Data from : Taipei Medical University

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Nestin with IR8-23 at 1/100.

Image

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IR8-23 anti- Nestin antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR8-23 anti-Nestin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

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