Overview

Product Name

Tau antibody

Product Number

IR39-123

Gene Description

microtubule-associated protein tau

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:3000 – 1:5000

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:200 – 1:300

Immunohistochemistry (Frozen) 1:200 – 1:400

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.12 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR39-123 anti-Tau antibody WB image

IR39-123 anti-Tau antibody IHC image

IR39-123 anti-Tau antibody IF image

All lanes : Anti-Tau antibody

Lysates/proteins at 60 μg per lane.

This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR39-123 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human astroglioma tissue labeling Tau with IR39-123 at 1/300.

Immunofluorescent analysis.

Sample : primary cortical neurons

Tau antibody (IR39-123) : 1-300

Anti-rabbit 488 : 1-500

Fixed :  4% paraformaldehyde at RT for 20 min.

IR39-123 anti-Tau antibody WB image

IR39-123 anti-Tau antibody ICC/IF image

IR39-123 anti-Tau antibody IHC image

All lanes : Anti-Tau antibody

Lysates/proteins at 45 μg per lane.

This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR39-123 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR39-123 anti-Tau antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical of frozen sections.

Sample : mouse cerebellum.

Green : Tau antibody (IR39-123) : 1-300

Anti-rabbit 488 : 1-500

DAPI (blue) was used as the nuclear counter stain.

IR39-123 anti-Tau antibody IHC image

IR39-123 anti-Tau antibody IF image

 Immunohistochemical analysis of paraffin embedded Human brain tissue labeling Tau with IR39-123 at 1/200.

Immunofluorescent analysis.

Sample : primary cortical neurons

Tau antibody (IR39-123) : 1-300

Anti-rabbit 488 : 1-500

Fixed :  4% paraformaldehyde at RT for 20 min.