IR39-123 Tau / MAPT antibody
Host :Rabbit
Clonality :Polyclonal
Reactivity :Human, Mouse, Rat
Package : 100 μl, 25μl
Overview |
|||
Product Name |
Tau antibody |
||
Product Number |
IR39-123 |
||
Gene Description |
microtubule-associated protein tau |
||
Clonality |
Polyclonal |
||
Host |
Rabbit |
||
Species Reactivity |
Human, mouse, rat |
||
Recommended Applications Dilutions |
Western Blot 1:3000 – 1:5000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:200 – 1:300 Immunohistochemistry (Frozen) 1:200 – 1:400 |
||
Storage Buffer |
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
||
Concentration |
1.12 mg/ml |
||
Purity |
Affinity column purified |
||
Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
||
Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
||
IR39-123 anti-Tau antibody WB image |
IR39-123 anti-Tau antibody IHC image |
IR39-123 anti-Tau antibody IF image |
|
|
|
|
|
All lanes : Anti-Tau antibody Lysates/proteins at 60 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR39-123 overnight at 4°C. |
Immunohistochemical analysis of paraffin embedded Human astroglioma tissue labeling Tau with IR39-123 at 1/300. |
Immunofluorescent analysis. Sample : primary cortical neurons Tau antibody (IR39-123) : 1-300 Anti-rabbit 488 : 1-500 Fixed : 4% paraformaldehyde at RT for 20 min. |
IR39-123 anti-Tau antibody WB image |
IR39-123 anti-Tau antibody ICC/IF image |
IR39-123 anti-Tau antibody IHC image |
|
|
|
|
|
All lanes : Anti-Tau antibody Lysates/proteins at 45 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR39-123 overnight at 4°C. |
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR39-123 anti-Tau antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain. |
Immunohistochemical of frozen sections.
Sample : mouse cerebellum. Green : Tau antibody (IR39-123) : 1-300 Anti-rabbit 488 : 1-500 DAPI (blue) was used as the nuclear counter stain. |
|
IR39-123 anti-Tau antibody IHC image |
IR39-123 anti-Tau antibody IF image |
||
|
|
||
Immunohistochemical analysis of paraffin embedded Human brain tissue labeling Tau with IR39-123 at 1/200. |
Immunofluorescent analysis. Sample : primary cortical neurons Tau antibody (IR39-123) : 1-300 Anti-rabbit 488 : 1-500 Fixed : 4% paraformaldehyde at RT for 20 min. |