Overview

Product Name

LRRK2 antibody

Product Number

IR38-119

Gene Description

leucine-rich repeat kinase 2

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications

Dilutions

Western Blot 1:500

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:100

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.59 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR38-119 anti-LRRK2 antibody WB image

IR38-119 anti-LRRK2 antibody IHC image

Anti-LRRK2 antibody at 1/500 dilution

Lysates/proteins at 30 μg per lane, This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3%BSA for an hour before being incubated with IR38-119 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human brain tissue labeling LRRK2 with IR38-119 at 1/100.

Image

IR38-119 anti-LRRK2 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR38-119 anti-LRRK2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.