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Home Products Primary antibody Apoptosis / Caspase IR101-420 PARP cleaved antibody

IR101-420 PARP cleaved antibody

Host :Rabbit
Clonality :Polyclonal
Reactivity :Human, Mouse, Rat
Package : 100 μl, 25μl
Blocking : 3%BSA
MW (kDa): 89, 115

Overview

Product Name

PARP cleaved antibody

Product Number

IR101-420

Gene Description

poly (ADP-ribose) polymerase 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human , mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500

Immunofluorescence 1:100 – 1:300

Immunohistochemistry (Paraffin) 1:100

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.4 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IR101-420 anti-PARP cleaved antibody WB image

All lanes : Anti-PARP celaved antibody - Loading Control at 1/500 dilution

 

Lysates/proteins at 30 μg per lane

This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with IR101-420 overnight at 4°C.

  

Image

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IR101-420 anti-PARP cleaved antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR101-420 anti- PARP cleaved antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

             

Image

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IR101-420 anti-PARP cleaved antibody ICC/IF image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling PARP1 cleaved with IR101-420 at 1/100.

Apoptosis / Caspase 1237301