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IR109_446 COX2 antibody3
IR109_446

IR109_446 COX2 antibody

Host :Rabbit
Clonality :Polyclonal
Reactivity :Human, Mouse, Rat
Package : 100 μl, 25μl

Overview

Product Name

COX2 antibody

Product Number

IR109-446

Gene Description

prostaglandin-endoperoxide synthase 2

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human , mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500 - 1:1000

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:100 – 1:200

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.05 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IR109-446 anti-COX2 antibody WB image

All lanes : Anti-COX2 antibody - at 1/1000 dilution

 

Lysates/proteins at 50 μg per lane.

This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR109-446 overnight at 4°C.

Image

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IR109-446 anti-COX2 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR109-446 anti-COX2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Image

IR109-446 anti- COX2 antibody IHC image

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Immunohistochemical analysis of paraffin embedded Human breast, testis, colorectal and lympho node cancer tissue labeling COX2 with IR109-446 at 1/100.

Inflammation 1237304
Attachments:
IR109_446_COX2.pdf