Home
1
Products
2
IR108-442 VEGF antibody3
IR108-442

IR108-442 VEGF antibody

Host : Rabbit
Clonality : Polyclonal
Reactivity : Human, Mouse, Rat
Package : 100 μl, 25 μl

Overview

Product Name

VEGF antibody

Product Number

IR108-442

Gene Description

Vascular endothelial growth factor A

Specificity

Detects the 121, 165 and 189 VEGF isoforms

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:5001:1000

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:100 – 1:300

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.56 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

8168abd8d0c54b2d722db0a189f7acd5.png

IR108-442 anti-VEGF antibody WB image

All lanes : Anti-VEGF antibody at 1/1000 dilution

 

Lysates/proteins at 30 μg per lane

This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR108-442 overnight at 4°C.

Image

5539ab4fe00c01532cf59252bf91eafa.png

IR108-442 anti-VEGF antibody WB image

All lanes : Anti-VEGF antibody at 1/500 dilution

 

Lysates/proteins at 60 μg per lane

This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR108-442 overnight at 4°C.

Image

06c47ac9d98589433cbb743acf6a818c.png

IR108-442 anti-VEGF antibody IHC image

Immunohistochemical analysis of paraffin embedded Human liver, breast, Colorental and kidney cancer tissue labeling VEGF with IR108-442 at 1/100.

Image

0eae9cf3b80a4d1f306da1a1898d3513.png

IR108-442 anti-VEGF antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR108-442 anti-VEGF antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Angiogenesis Factor 1237306
Attachments:
IR108-442_VEGF.pdf