Overview

Product Name

CD34 antibody

Product Number

IR76-259

Gene Description

CD34 molecule

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human , mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500

Immunofluorescence 1:100 – 1:200

Immunohistochemistry (Paraffin) 1:50 – 1:100

Storage Buffer

100mM Tris Glycine, 1%BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.71 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

IR76-259 anti-CD34 antibody WB image

All lanes : Anti-CD34 antibody - at 1/500 dilution

Lysates/proteins at 30 μg per lane

This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA for an hour before being incubated with IR76-259 overnight at 4°C.

Image

IR76-259 anti-CD34 antibody I image

Immunohistochemical analysis of paraffin embedded Human kidney cancer tissue labeling CD34 with IR76-259 at 1/100.

Image

IR76-259 anti-CD34 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR76-259 anti- CD34 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.