IR211-769 EGFR
Host : Rabbit
Clonality : Polyclonal
Reactivity : Human, Mouse, Rat
Package : 100 μl, 25 μl
MW (kDa): 120-180
Catalog Number: IR211-769 Anti-EGFR antibody |
Package size: 25, 100 μl Store at: -20°C MW (kDa): 120-180 |
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Overview |
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Product Name |
EGFR antibody |
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Product Number |
IR211-769 |
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Gene Description |
epidermal growth factor receptor |
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Clonality |
Polyclonal |
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Host |
Rabbit |
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Species Reactivity |
Human , mouse, rat |
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Recommended Applications Dilutions |
Western Blot 1:2000 - 1:3000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:200 – 1:500 |
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Storage Buffer |
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
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Concentration |
1.31 mg/ml |
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Purity |
Affinity column purified |
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Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
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Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
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IR211-769 anti-EGFR antibody WB image |
IR211-769 anti-EGFR antibody IHC image |
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Anti-EGFR antibody - at 1/2000 dilution Lysates at 30 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR211-769 overnight at 4°C. |
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling EGFR with IR211-769 at 1/300. |
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IR211-769 anti-EGFR antibody ICC/IF image |
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR211-769 anti-EGFR antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain. |
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Customer feedback Image |
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IR211-769 anti-EGFR antibody IHC image |
Immunohistochemical of paraffin embedded.
Sample : human breast carcinoma. EGFR antibody (IR211-769) : 1-300 |