IR218-1 EpCAM antibody
Host : Rabbit
Clonality : Polyclonal
Reactivity : Human, Mouse, Rat
Package : 100 μl, 25 μl
Overview |
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Product Name |
EpCAM antibody |
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Product Number |
IR218-1 |
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Gene Description |
epithelial cell adhesion molecule |
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Clonality |
Polyclonal |
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Host |
Rabbit |
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Species Reactivity |
Human, mouse, rat |
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Recommended Applications Dilutions |
Western Blot 1:1000 – 1:2000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:200 – 1:300 |
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Storage Buffer |
100mM Tris Glycine, 1%BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
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Concentration |
0.88 mg/ml |
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Purity |
Affinity column purified |
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Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
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Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
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Image |
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IR218-1 anti-EpCAM antibody WB image |
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All lanes : Anti-EpCAM antibody - at 1/1000 dilution
Lysates/proteins at 40 μg per lane This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane ws then blocked for an hour before being incubated with IR218-1 overnight at 4°C. |
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Image |
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R218-1 anti-EpCAM antibody ICC/IF image |
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR218-1 anti-EpCAM antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain. |
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R218-1 anti-EpCAM antibody IHC image |
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling EpCAM with IR218-1 at 1/200. |