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IR127-515 IRE1 alpha / ERN1 antibody3
Home Products Primary antibody ER stress IR127-515 IRE1 alpha / ERN1 antibody
IR127-515

IR127-515 IRE1 alpha / ERN1 antibody

Host : Rabbit
Clonality : Polyclonal
Reactivity : Human, Mouse, Rat
Package : 100 μl, 25 μl

Overview

Product Name

IRE1 alpha antibody

Product Number

IR127-515

Gene Description

endoplasmic reticulum to nucleus signaling 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:200 – 1:400

Immunohistochemistry (Paraffin) 1:100 – 1:150

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.91 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR127-515 anti-IRE1 alpha antibody WB image

IR127-515 anti-IRE1 alpha antibody IHC image

IR127-515 anti-IRE1 alpha antibody ICC/IF image

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Anti-IRE1 alpha antibody - at 1/500 dilution, Lysates at 50 μg per lane.

This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR127-515 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded mouse testis labeling IRE1 alpha with IR127-515 at 1/100.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with IR127-515 anti- IRE1 alpha antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

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