p63 : Squamous, Basal & Myoepithelial Cell Marker ( IR205-742 p63 antibody )
Host : Rabbit
Clonality : Polyclonal
Reactivity : Human, Mouse, Rat
Package : 100 μl, 25 μl
MW (kDa): 72-80
App : WB, ICC/IF, IHC
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Overview |
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Product Name |
P63 antibody |
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Product Number |
IR205-742 |
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Gene Description |
tumor protein p63 |
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Clonality |
Polyclonal |
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Host |
Rabbit |
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Species Reactivity |
Human, Mouse, Rat |
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Recommended Applications Dilutions |
Western Blot 1:500 – 1:1000 Immunofluorescence 1:100 – 1:300 Immunohistochemistry (Paraffin) 1:100 |
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Storage Buffer |
100mM Tris Glycine, 1%BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
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Concentration |
0.36 mg/ml |
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Purity |
Affinity column purified |
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Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
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Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
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IR205-742 anti- p63 antibody WB image |
Customer feedback IR205-742 anti- p63 antibody IHC image |
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All lanes : Anti-p63 antibody - at 1/500 dilution Lysates/proteins at 50 μg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3%BSA an hour before being incubated with IR205-742 overnight at 4°C. |
Immunohistochemical analysis of paraffin embedded mouse tissue labeling p63 with IR205-742 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0 Data from: Li-Tzung Biotechnology. Co |
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IR205-742 anti- p63 antibody ICC/IF image |
Customer feedback IR205-742 anti- p63 antibody IHC image |
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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR205-742 anti-p63 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain. |
Immunohistochemical analysis of paraffin embedded Human tissue labeling p63 with IR205-742 at 1/100. |
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Customer feedback IR205-742 anti- p63 antibody IHC image |
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Immunohistochemical analysis of paraffin embedded Human breast cancer tissue labeling p63 with IR205-742 at 1/100. |
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Immunohistochemical analysis of paraffin embedded mouse tissue labeling p63 with IR205-742 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0 Data from: Li-Tzung Biotechnology. Co |
