IR205-742 P63 antibody
Host : Rabbit
Clonality : Polyclonal
Reactivity : Human, Mouse, Rat
Package : 100 μl, 25 μl
Blocking : 3%BSA
MW (kDa): 72-80
Overview | ||
Product Name | P63 antibody | |
Product Number | IR205-742 | |
Gene Description | tumor protein p63 | |
Clonality | Polyclonal | |
Host | Rabbit | |
Species Reactivity | Human, Mouse, Rat | |
Recommended Applications Dilutions | Western Blot 1:500 – 1:1000 Immunofluorescence 1:100 – 1:300 Immunohistochemistry (Paraffin) 1:100 | |
Storage Buffer | 100mM Tris Glycine, 1%BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative | |
Concentration | 0.36 mg/ml | |
Purity | Affinity column purified | |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C | |
Notes | Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. | |
Image | ||
IR205-742 anti-p63 antibody WB image | ||
All lanes : Anti-p63 antibody - at 1/500 dilution
Lysates/proteins at 50 μg per lane This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3%BSA an hour before being incubated with IR205-742 overnight at 4°C. | ||
Image | |
IR205-742 anti- p63 antibody IHC image | |
Immunohistochemical analysis of paraffin embedded Human colorectal and liver cancer tissue labeling p63 with IR205-742 at 1/100. | |
Image | |
IR205-742 anti- p63 antibody ICC/IF image | |
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR205-742 anti-p63 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain. |