Overview

Product Name

Estrogen Receptor alpha antibody

Product Number

IR213-777

Gene Description

estrogen receptor 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:100 – 1:200

Immunohistochemistry (Paraffin) 1:200 – 1:250

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.38 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

IR213-777 anti- Estrogen Receptor alpha antibody WB image

All lanes : Anti- Estrogen Receptor alpha antibody at 1/1000 dilution

Lysates/proteins at 60 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR213-777 overnight at 4°C.

Image

IR213-777 anti- Estrogen Receptor alpha antibody IHC image

Immunohistochemical analysis of paraffin embedded Human breast and colorectal cancer tissue labeling Estrogen Receptor alpha with IR213-777 at 1/200.