IR213-777 Estrogen Receptor alpha / ER alpha antibody
Host : Rabbit
Clonality : Polyclonal
Reactivity : Human, Mouse, Rat
Package : 100 μl, 25 μl
MW (kDa): 66-60
Overview | ||
Product Name | Estrogen Receptor alpha antibody | |
Product Number | IR213-777 | |
Gene Description | estrogen receptor 1 | |
Clonality | Polyclonal | |
Host | Rabbit | |
Species Reactivity | Human, Mouse, Rat | |
Recommended Applications Dilutions | Western Blot 1:500 – 1:1000 Immunofluorescence 1:100 – 1:200 Immunohistochemistry (Paraffin) 1:200 – 1:250 | |
Storage Buffer | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative | |
Concentration | 1.38 mg/ml | |
Purity | Affinity column purified | |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C | |
Notes | Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. | |
Image | ||
IR213-777 anti- Estrogen Receptor alpha antibody WB image | ||
All lanes : Anti- Estrogen Receptor alpha antibody at 1/1000 dilution
Lysates/proteins at 60 μg per lane This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR213-777 overnight at 4°C. | ||
Image |
IR213-777 anti- Estrogen Receptor alpha antibody IHC image |
Immunohistochemical analysis of paraffin embedded Human breast and colorectal cancer tissue labeling Estrogen Receptor alpha with IR213-777 at 1/200. |