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Home Products Primary antibody ER stress IR136-543 NRF2 antibody

IR136-543 NRF2 antibody

Host : Rabbit
Clonality : Polyclonal
Reactivity : Human, Mouse, Rat
Package : 100 μl, 25 μl
MW (kDa): 125-160

Overview

Product Name

NRF2 antibody

Product Number

IR136-543

Gene Description

nuclear factor, erythroid 2-like 2

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:100 – 1:200

Immunohistochemistry (Paraffin) 1:50 – 1:100

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.41 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IR136-543 anti-NRF2 antibody WB image

All lanes : Anti-NRF2 antibody at 1/800 dilution

 

Lysates/proteins at 30 μg per lane

This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR136-543 overnight at 4°C.

 

  

Image

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IR136-543 anti-NRF2 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR136-543 anti-NRF2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Image

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IR136-543 anti-NRF2 antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with IR136-543 at 1/100.

ER stress 1257602
Attachments:
IR136-543_NRF2.pdf