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IR47-146 α Smooth Muscle Actin antibody3
Home Products Primary antibody EMT Marker IR47-146 α Smooth Muscle Actin antibody
IR47-146

IR47-146 α Smooth Muscle Actin antibody

Host : Rabbit
Clonality : Polyclonal
Reactivity : Human, Mouse, Rat
Package : 100 μl, 25 μl
MW (kDa): 43

Overview

Product Name

α Smooth Muscle Actin antibody

Product Number

IR47-146

Gene Description

actin, alpha 2, smooth muscle, aorta

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:1000 – 1:3000

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:100 – 1:400

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.61 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR47-146 anti-α Smooth Muscle Actin antibody WB image

IR47-146 anti-α Smooth Muscle Actin antibody IHC image

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Anti-α Smooth Muscle Actin antibody at 1/2000 dilution

Lysates at 35 μg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR47-146 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling α Smooth Muscle Actin with IR47-146 at 1/200.


IR47-146 anti-α Smooth Muscle Actin antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR47-146 anti- α Smooth Muscle Actin antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

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IReal Biotechnology Co., Ltd.               Website : www.irealbio.com                TEL : 03-526-0005               E-mail : irealbio@irealbio.com

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