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IR212-772 Ki-67 antibody3
IR212-772

IR212-772 Ki-67 antibody

Host : Rabbit
Clonality : Polyclonal
Reactivity : Human
Package : 100 μl, 25 μl
Blocking : 3%BSA

Overview

Product Name

Ki-67 antibody

Product Number

IR212-772

Gene Description

marker of proliferation Ki-67

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:100 – 1:200

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IR212-772 anti- Ki-67 antibody WB image

All lanes : Anti- Ki-67 antibody - at 1/500 dilution

 

Lysates/proteins at 50 μg per lane

This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked with 3% BSA an hour before being incubated with IR212-772 overnight at 4°C.

 

 

IR212-772 anti- Ki-67 antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Ki-67 with IR212-772 at 1/100.

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Image

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IR212-772 anti- Ki-67 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR212-772 anti- Ki-67 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Cancer Marker 1299041
Attachments:
IR212-772_Ki-67.pdf