IR226-865 Cytokeratin 5/6, CK5/6 antibody
Host : Rabbit
Clonality : Polyclonal
Reactivity : Human
Package : 100 μl, 25 μl
Overview | ||
Product Name | Cytokeratin 5/6 antibody | |
Product Number | IR226-865 | |
Gene Description | keratin 5, type II | |
Clonality | Polyclonal | |
Host | Rabbit | |
Species Reactivity | Human | |
Recommended Applications Dilutions | Western Blot 1:1000 – 1:2000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:100 – 1:300 | |
Storage Buffer | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative | |
Concentration | 1.74 mg/ml | |
Purity | Affinity column purified | |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C | |
Notes | Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. | |
Image | ||
| IR226-865 anti-Cytokeratin 5/6 antibody WB image | |
All lanes : Anti-Cytokeratin 5/6 antibody - at 1/2000 dilution
Lysates/proteins at 45 μg per lane This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR226-865 overnight at 4°C. | ||
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Image | |
| IR226-865 anti-Cytokeratin 5/6 antibody WB image |
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 5/6 with IR226-865 at 1/100. | |
| IR226-865 anti-Cytokeratin 5/6 antibody ICC/IF image |
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR226-865 anti- Cytokeratin5/6 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain. |
