Overview

Product Name

Cytokeratin 5/6 antibody

Product Number

IR226-865

Gene Description

keratin 5, type II

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Recommended

Applications Dilutions

Western Blot 1:1000 – 1:2000

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:100 – 1:300

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.74 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

IR226-865 anti-Cytokeratin 5/6 antibody WB image

All lanes : Anti-Cytokeratin 5/6 antibody - at 1/2000 dilution

Lysates/proteins at 45 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR226-865 overnight at 4°C.

Image

IR226-865 anti-Cytokeratin 5/6 antibody WB image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 5/6 with IR226-865 at 1/100.

IR226-865 anti-Cytokeratin 5/6 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR226-865 anti- Cytokeratin5/6 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.