IR236-890 Cytokeratin 18/19, CK18/19 antibody
Host : Rabbit
Clonality : Polyclonal
Reactivity : Human
Package : 100 μl, 25 μl
Overview | ||
Product Name | Cytokeratin 18/19 antibody | |
Product Number | IR236-890 | |
Gene Description | keratin 18, type I | |
Clonality | Polyclonal | |
Host | Rabbit | |
Species Reactivity | Human | |
Recommended Applications Dilutions | Western Blot 1:1000 – 1:2000 Immunofluorescence 1:200 – 1:500 Immunohistochemistry (Paraffin) 1:100 – 1:300 | |
Storage Buffer | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative | |
Concentration | 1.77 mg/ml | |
Purity | Affinity column purified | |
Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C | |
Notes | Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. | |
Image | ||
![]() | IR236-890 anti-Cytokeratin 18/19 antibody WB image | |
All lanes : Anti-Cytokeratin 18/19 antibody - at 1/2000 dilution
Lysates/proteins at 45 μg per lane This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR236-890 overnight at 4°C. | ||
I
IR236-890 anti-Cytokeratin 18/19 antibody IHC image |
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 18/19 with IR236-890 at 1/200. |
IR236-890 anti-Cytokeratin 18/19 antibody ICC/IF image | |
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR236-890 anti- Cytokeratin 18/19 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain. |