Overview

Product Name

ERK1+ERK2 phospho (Thr202 / Tyr204) antibody

Product Number

IR188-706

Gene Description

mitogen-activated protein kinase 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human , mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:200 – 1:500

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.73 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

IR188-706 anti-ERK1+ERK2 phospho (Thr202 / Tyr204) antibody WB image

All lanes : Anti-ERK1+ERK2 phospho (Thr202 / Tyr204) antibody at 1/800 dilution

Lysates/proteins at 40 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR188-706 overnight at 4°C.

Image

IR188-706 anti-ERK1+ERK2 phospho (Thr202 / Tyr204) antibody WB image

Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody (IR188-706).