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Pan-Cytokeratin, Pan CK antibody3
Home Products Primary antibody Cancer Marker Pan-Cytokeratin, Pan CK antibody
IR222-861

Pan-Cytokeratin, Pan CK antibody

Host : Rabbit
Clonality : Polyclonal
Reactivity : Human
Package : 100 μl, 25 μl
MW (kDa): 43-55

Overview

Product Name

Pan-Cytokeratin antibody

Product Number

IR222-861

Gene Description

keratin 77, type II

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Specificity

Detects endogenous levels of total keratin 5, 6, 7, 14, 17, 18, 19 and 20. The antibody does not cross-react with other keratins.

Recommended

Applications Dilutions

Western Blot 1:2000 – 1:5000

Immunofluorescence 1:300 – 1:500

Immunohistochemistry (Paraffin) 1:300 – 1:500

Storage Buffer

PBS (pH 7.4), 40% Glycerol. 0.025% ProClin 300 was added as a preservative

Concentration

. mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IR222-861 anti-pan Cytokeratin antibody WB image

All lanes : Anti-pan Cytokeratin antibody - at 1/2000 dilution

 

Lysates/proteins at 45 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR222-861 overnight at 4°C.

IR222-861 anti-pan Cytokeratin antibody WB image

All lanes : Anti-pan Cytokeratin antibody - at 1/2000 dilution

Lysates/proteins at 45 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR222-861 overnight at 4°C.

22137e310759ccbd573b86bb5be3c3e4.png

Image

023536fd5e082986ea5407e2b9fc808b.jpg

IR222-861 anti-pan Cytokeratin antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling pan Cytokeratin with IR222-861 at 1/400.

Image

004b21526484a8940fcf5a0c2686da0f.png

IR222-861 anti-pan Cytokeratin antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR222-861 anti-pan Cytokeratin0 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

IReal Biotechnology Co., Ltd.

7F., No. 9, Sec. 3, Zhonghua Rd., North Dist., Hsinchu City 300, Taiwan (R.O.C.)

Website : www.irealbio.com      TEL : 03-526-0005       E-mail : irealbio@irealbio.com

Cancer Marker 1328393