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IR74-P1 IL6 antibody 3
IR74-P1_IL6

IR74-P1 IL6 antibody

Overview

Product Name

IL6 antibody

Product Number

IR74-P1

Gene Description

interleukin 6

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Recommended

Applications Dilutions

Western Blot 1:500 - 1:700

Immunofluorescence 1:300 – 1:400

Immunohistochemistry (Paraffin) 1:100 – 1:200

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.04 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

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IR74-P1 anti-IL6 antibody WB image

All lanes : Anti-IL6 beta antibody - at 1/500 dilution

 

Lysates/proteins at 70 μg per lane.

This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR74-P1 overnight at 4°C.

Image

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IR74-P1 anti-IL6 antibody WB image

Western blot analysis of recombinant Human Interleukin-6 (hHis6IL-6) using IL-6 antibody (IR74-P1).

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IR74-P1 anti-IL6 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR74-P1 anti- IL-6 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Image

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IR74-P1 anti-IL6 antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling IL6 with IR74-P1 at 1/200.

Inflammation 1424443
Attachments:
IR74_P1_IL6.pdf