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IR106-P2 TNF alpha antibody3
Home Products Primary antibody Inflammation IR106-P2 TNF alpha antibody
IR106-P2

IR106-P2 TNF alpha antibody

Overview

Product Name

TNF alpha antibody

Product Number

IR106-P2

Gene Description

tumor necrosis factor

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse

Recommended

Applications Dilutions

Western Blot 1:500-1:1500

Immunofluorescence 1:200 – 1:400

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IR106-P2 anti-TNF alpha antibody WB image

All lanes : Anti- TNF alpha antibody at 1/700 dilution

 

Lysates/proteins at 50 μg per lane

This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR106-P2 overnight at 4°C.

Image

1ec2c4310d87dae61df3387b662d3583.jpg

IR106-P2 anti-TNF alpha antibody WB image

TNF alpha antibody detects His-tagged recombinant human TNF alpha protein by western blot analysis. The membrane was then blocked for an hour before being incubated with IR106-P2 overnight at 4°C.

TNF alpha antibody (IR106-P2) diluted at 1:3000.

Image

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IR106-P2 anti-TNF alpha antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR106-P2 anti- TNF alpha antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Inflammation 1424447
Attachments:
IR106-P2_TNF_alpha.pdf