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IR23-P1, IR23-P2 Neurofilament-L / NF-L antibody3
Home Products Primary antibody IR23-P1, IR23-P2 Neurofilament-L / NF-L antibody
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IR23-P1, IR23-P2 Neurofilament-L / NF-L antibody

Overview

Product Name

Neurofilament-L / NF-L antibody

Product Number

IR23-P1

Gene Description

neurofilament, light polypeptide

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:1000– 1:2000

Immunofluorescence 1:200 – 1:500

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.9 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IR23-P1 anti- Neurofilament-L / NF-L antibody WB image

All lanes : Anti- Neurofilament-L / NF-L antibody at 1/1000 dilution

 

Lysates/proteins at 60 μg per lane

This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR23-P1 overnight at 4°C.

 

Customer feedback Image

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IR23-P1 anti- Neurofilament-L / NF-L antibody IF image

Immunofluorescent analysis.

 

Sample: primary cortical neurons

NF-L antibody (IR23-P1): 1-250

Red: Tuj1 antibody

Anti-rabbit 488: 1-500

Fixed: 4% paraformaldehyde at RT for 20 min.

Customer feedback Image

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R23-P1 anti- Neurofilament-L / NF-L antibody IHC image

Immunohistochemical analysis of paraffin embedded Human brain tissue labeling Neurofilament-L / NF-L with IR23-P1 at 1/100.

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