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IR3-8 GAPDH antibody3
Home Products Primary antibody IR3-8 GAPDH antibody
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IR3-8 GAPDH antibody

Overview

Product Name

GAPDH antibody

Product Number

IR3-8

Gene Description

glyceraldehyde-3-phosphate dehydrogenase

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:5000 - 1:10000

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:400 – 1:600

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.66 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR3-8 anti-GAPDH antibody WB image

IR3-8 anti-GAPDH antibody ICC/IF image

IR3-8 anti-GAPDH antibody IHC image

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All lanes : Anti-GAPDH antibody - Loading Control at 1/5000 dilution

Lysates/proteins at 20 μg per lane

This blot was produced using a 10% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with IR3-8 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR3-8 anti-GAPDH antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse testis labeling GAPDH with IR3-8 at 1/400.

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Attachments:
IR3-8_GAPDH.pdf