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IR4-10 Histone H3 antibody3
Home Products Primary antibody IR4-10 Histone H3 antibody
IR4-10

IR4-10 Histone H3 antibody

Overview

Product Name

Histone H3 antibody

Product Number

IR4-10

Gene Description

histone cluster 1, H3a

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human , mouse, rat

Recommended

Applications Dilutions

Western Blot 1:1000 - 1:3000

Immunofluorescence 1:200 – 1:500

Immunohistochemistry (Paraffin) 1:200 – 1:400

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.39 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IR4-10 anti-Histone H3 antibody WB image

All lanes : Anti-Histone H3 antibody - at 1/1000 dilution

 

Lysates/proteins at 50 μg per lane.

This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR4-10 overnight at 4°C.

Image

bc2c99d4e4bc43a50123b464d5ce24f3.jpg

IR4-10 anti-Histone H3 antibody WB image

All lanes : Anti-Histone H3 antibody - at 1/2000 dilution

 

Lysates/proteins at 50 μg per lane.

This blot was produced using a 15% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR4-10 overnight at 4°C.

Image

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IR4-10 anti- Histone H3 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR4-10 anti-Histone H3 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Customer feedback Image

48be5162a8f6e2df6d744eae4287abb5.jpg

IR4-10 anti- Histone H3 antibody IHC image

Immunohistochemical of Paraffin-embedded.

 

Sample : mouse duodenum and brain.

Histone H3 antibody (IR4-10) : 1-300

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Attachments:
IR4-10_Histone_H3.pdf