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IRT003 DYKDDDK Tag antibody3
Home Products Primary antibody IRT003 DYKDDDK Tag antibody
IRT003

IRT003 DYKDDDK Tag antibody

Overview

Product Name

DYKDDDDK-tag antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody)

Product Number

IRT003

Clonality

Polyclonal

Host

Rabbit

Immunogen

Synthetic peptide (DYKDDDDK)

Recommended

Applications Dilutions

Western Blot 1:3000-1:5000

Immunofluorescence 1:500 – 1:1000

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IRT003 anti DYKDDDDK-tag antibody WB image

All lanes : Anti DYKDDDDK-tag antibody at 1/3000 dilution

 

Lysates/proteins at 35 μg per lane

This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IRT003 overnight at 4°C.

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