Overview

Product Name

AKT (pan) antibody

Product Number

IR171-666

Gene Description

v-akt murine thymoma viral oncogene homolog

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:1000 – 1:2000

Immunofluorescence 1:200 – 1:500

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.93 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

IR171-666 anti-AKT (pan) antibody WB image

All lanes : Anti-AKT (pan) antibody at 1/1000 dilution

Lysates/proteins at 30 μg per lane

This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR171-666 overnight at 4°C.

Image

IR171-666 anti-AKT (pan) antibody WB image

All lanes : Anti-AKT (pan) antibody at 1/1000 dilution

Lysates/proteins at 60 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR171-666 overnight at 4°C.

        Image

IR171-666 anti-AKT (pan) antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR171-666 anti- AKT (pan) antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.