Home
1
Products
2
IR82-282 eNOS / NOS3 antibody ( endothelial marker )3
Home Products Primary antibody IR82-282 eNOS / NOS3 antibody ( endothelial marker )
IR82-282

IR82-282 eNOS / NOS3 antibody ( endothelial marker )

Overview

Product Name

eNOS / NOS3 antibody

Product Number

IR82-282

Gene Description

nitric oxide synthase 3 (endothelial cell)

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:1000 – 1:2000

Immunofluorescence 1:200 – 1:400

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

d64e8f090c2066c87a3c28863b0b69f4.jpg

IR82-282 anti-eNOS / NOS3 antibody WB image

All lanes : Anti- eNOS / NOS3 antibody - at 1/1000 dilution

 

Lysates/proteins at 40 μg per lane

This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with IR82-282 overnight at 4°C.

IR82-282 anti-eNOS / NOS3 antibody IHC image

IR82-282 anti-eNOS / NOS3 antibody ICC/IF image

bbd62db0202f823c7b0ebe850af25b45.jpg

a1317f780a682edce08db5a5a6b72ecc.jpg

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling eNOS with IR82-282 at 1/200.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR82-282 anti- eNOS/ NOS3 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

 

1439519
Attachments:
IR82-282_eNOS_NOS3.pdf