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IR187-705 ERK1+ERK2 antibody3
Home Products Primary antibody IR187-705 ERK1+ERK2 antibody
IR187-705

IR187-705 ERK1+ERK2 antibody

Overview

Product Name

ERK1+ERK2 antibody

Product Number

IR187-705

Gene Description

mitogen-activated protein kinase 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human , mouse, rat

Recommended

Applications Dilutions

Western Blot 1:1000– 1:3000

Immunofluorescence 1:300 – 1:500

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.32 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IR187-705 anti-ERK1+ERK2 antibody WB image

All lanes : Anti-ERK1+ERK2 antibody at 1/2000 dilution

Lysates/proteins at 30 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR187-705 overnight at 4°C.

Image

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IR187-705 anti-ERK1+ERK2 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR187-705 anti-ERK1+ERK2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

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