IR187-705 ERK1+ERK2 antibody
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Overview |
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Product Name |
ERK1+ERK2 antibody |
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Product Number |
IR187-705 |
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Gene Description |
mitogen-activated protein kinase 1 |
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Clonality |
Polyclonal |
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Host |
Rabbit |
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Species Reactivity |
Human , mouse, rat |
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Recommended Applications Dilutions |
Western Blot 1:1000– 1:3000 Immunofluorescence 1:300 – 1:500 |
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Storage Buffer |
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
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Concentration |
1.32 mg/ml |
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Purity |
Affinity column purified |
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Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
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Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
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Image |
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IR187-705 anti-ERK1+ERK2 antibody WB image |
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All lanes : Anti-ERK1+ERK2 antibody at 1/2000 dilution Lysates/proteins at 30 μg per lane This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR187-705 overnight at 4°C. |
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Image |
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IR187-705 anti-ERK1+ERK2 antibody ICC/IF image |
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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR187-705 anti-ERK1+ERK2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain. |
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