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IR2-7 Beta Actin antibody3
Home Products Primary antibody IR2-7 Beta Actin antibody
IR2-7

IR2-7 Beta Actin antibody

Overview

Product Name

Beta Actin antibody

Product Number

IR2-7

Gene Description

actin, beta

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:2000 - 1:5000

Immunofluorescence 1:600 – 1:700

Immunohistochemistry (Paraffin) 1:400 – 1:600

Storage Buffer

100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.41 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR2-7 anti-Beta Actin antibody WB image

IR2-7 anti-Beta Actin antibody ICC/IF image

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All lanes : Anti-Beta Actin antibody - at 1/2000 dilution

Lysates/proteins at 40 μg per lane.

This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR2-7 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR2-7 anti- Beta Actin antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Image

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IR2-7 anti-Beta Actin antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Beta Actin with IR2-7 at 1/600.

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Attachments:
IR2-7_Beta_Actin.pdf