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IR137-549 RELA, NF-κB p65 antibody3
Home Products Primary antibody IR137-549 RELA, NF-κB p65 antibody
IR137-549

IR137-549 RELA, NF-κB p65 antibody

Overview

Product Name

NF-kB p65 antibody

Product Number

IR137-549

Gene Description

v-rel avian reticuloendotheliosis viral oncogene homolog A

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:1000 – 1:2000

Immunofluorescence 1:200 – 1:500

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR137-549 anti- NF-kB p65 antibody WB image

IR137-549 anti- NF-kB p65 antibody ICC/IF image

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All lanes : Anti- NF-kB p65 antibody at 1/1000 dilution

 

Lysates/proteins at 30 μg per lane

This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR137-549 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR137-549 anti- NF-kB p65 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

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