Overview

Product Name

Phospho-AKT (Ser473) antibody

Product Number

IR172-668

Gene Description

v-akt murine thymoma viral oncogene homolog

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human , mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:200 – 1:400

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.55 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

IR172-668 anti-AKT (pan) antibody WB image

All lanes : Anti-AKT (pan) antibody at 1/800 dilution

Lysates/proteins at 60 μg per lane

This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR172-668 overnight at 4°C.