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IRM001 Alpha tubulin antibody3
Home Products Primary antibody IRM001 Alpha tubulin antibody
IRM001

IRM001 Alpha tubulin antibody

Overview

Product Name

Alpha tubulin [2-4D-8D] monoclonal antibody

Product Number

IRM001

Gene Description

tubulin, alpha class I

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:5000 - 1:10000

Immunofluorescence 1:200 – 1:500

ELISA 1:10000 - 1:30000

Storage Buffer

50mM Tris Glycine, 40% Glycerol (pH7.4). 0.025% ProClin 300 was added as a preservative

Concentration

1 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C. Avoid repeated freeze/thaw cycles.

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

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IRM001 anti-alpha Tubulin monoclonal antibody WB image

All lanes : Anti-alpha Tubulin monoclonal antibody - Loading Control at 1/8000 dilution

 

Lysates/proteins at 30 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IRM001 overnight at 4°C.

Image

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IRM001 anti-alpha Tubulin monoclonal antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IRM001 anti-alpha Tubulin monoclonal antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

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