IRM001 Alpha tubulin antibody
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Overview |
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Product Name |
Alpha tubulin [2-4D-8D] monoclonal antibody |
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Product Number |
IRM001 |
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Gene Description |
tubulin, alpha class I |
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Clonality |
Monoclonal |
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Host |
Mouse |
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Isotype |
IgG1 |
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Species Reactivity |
Human, mouse, rat |
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Recommended Applications Dilutions |
Western Blot 1:5000 - 1:10000 Immunofluorescence 1:200 – 1:500 ELISA 1:10000 - 1:30000 |
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Storage Buffer |
50mM Tris Glycine, 40% Glycerol (pH7.4). 0.025% ProClin 300 was added as a preservative |
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Concentration |
1 mg/ml |
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Purity |
Affinity column purified |
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Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C. Avoid repeated freeze/thaw cycles. |
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Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
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Image |
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IRM001 anti-alpha Tubulin monoclonal antibody WB image |
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All lanes : Anti-alpha Tubulin monoclonal antibody - Loading Control at 1/8000 dilution
Lysates/proteins at 30 μg per lane This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IRM001 overnight at 4°C. |
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Image |
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IRM001 anti-alpha Tubulin monoclonal antibody ICC/IF image |
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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IRM001 anti-alpha Tubulin monoclonal antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain. |
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