Home
1
Products
2
IR77-264 CD45 Intracellular antibody3
Home Products Primary antibody IR77-264 CD45 Intracellular antibody
IR77-264

IR77-264 CD45 Intracellular antibody

Overview

Product Name

CD45 antibody (Intracellular domain)

Product Number

IR77-264

Gene Description

protein tyrosine phosphatase, receptor type, C

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:400 – 1:800

Immunohistochemistry (Paraffin) 1:400 – 1:800

Flow Cytometry 1:50 – 1:150

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR77-264 anti-CD45 antibody WB image

IR77-264 anti-CD45 antibody FACS image

cf90193ca620619e7a1710a5252efdb9.jpg

4116f91223ad4a0c31bb3e8097b42cbc.jpg

All lanes : Anti-CD45 antibody at 1/1000 dilution

Lysates/proteins at 50 μg per lane.

This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR77-264 overnight at 4°C.

ProductCD45 antibody

CatalogIR77-264

Cell : THP-1

Data from : National Taiwan Ocean University

IR77-264 anti- CD45 antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD45 with IR77-264 at 1/400.

21fd3297ab6a9ccb31b12ec9040821c8.jpg

d6524fd8149c86b950b48b576b4d85b9.jpg

IR77-264 anti-CD45 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR77-264 anti-CD45 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

1439547
Attachments:
IR77-264_CD45_ICD.pdf