Overview

Product Name

CTGF antibody

Product Number

IR289-955

Gene Description

connective tissue growth factor

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:200 – 1:300

Immunohistochemistry (Paraffin) 1:100 – 1:150

Storage Buffer

100mM Tris Glycine, 40% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR289-955 anti-CTGF antibody WB image

IR289-955 anti-CTGF antibody ICC/IF image

Anti-CTGF antibody at 1/700 dilution

Lysates at 50 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR289-955 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR289-955 anti-CTGF antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Image

IR289-955 anti-CTGF antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CTGF with IR289-955 at 1/100.