IR252-897 CD3 antibody
Overview |
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Product Name |
CD3 antibody |
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Product Number |
IR252-897 |
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Gene Description |
CD3e molecule, epsilon (CD3-TCR complex) |
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Clonality |
Polyclonal |
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Host |
Rabbit |
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Species Reactivity |
Human |
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Recommended Applications Dilutions |
Western Blot 1:500 – 1:1000 Immunofluorescence 1:300 – 1:400 Immunohistochemistry (Paraffin) 1:300 – 1:500 Flow Cytometry 1:50 – 1:150 |
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Storage Buffer |
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
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Concentration |
0.69 mg/ml |
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Purity |
Affinity column purified |
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Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
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Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
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IR252-897 anti-CD3 antibody WB image |
IR252-897 anti-CD3 antibody FACS image |
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All lanes : Anti-CD3 antibody at 1/800 dilution
Lysates/proteins at 60 μg per lane This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR252-897 overnight at 4°C. |
Product:CD3 antibody Catalog:IR252-897 Cell : Jurkat PE labelled secondary antibody. Unlabelled sample (black) was used as a control. Data from : National Taiwan Ocean University |
IR252-897 anti-CD3 antibody IHC image |
IR252-897 anti-CD3 antibody ICC/IF image |
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Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD3 with IR252-897 at 1/400. |
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR252-897 anti- CD3 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain. |