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IR16-2 VDAC1 / Porin antibody3
Home Products Primary antibody IR16-2 VDAC1 / Porin antibody
IR16-2

IR16-2 VDAC1 / Porin antibody

Overview

Product Name

VDAC1 antibody

Product Number

IR16-2

Gene Description

voltage-dependent anion channel 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:500 - 1:1000

Immunofluorescence 1:200 – 1:400

Immunohistochemistry (Paraffin) 1:200 – 1:300

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.01 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR16-2 anti-VDAC1 antibody WB image

IR16-2 anti-VDAC1 antibody IHC image

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Anti-VDAC1 beta antibody - at 1/500 dilution.

Lysates at 60 μg per lane. This blot was produced using a 12% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR16-2 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling VDAC1 with IR16-2 at 1/150.

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IR16-2 anti-VDAC1 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR16-2 anti-VDAC1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

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Attachments:
IR16-2_VDAC1_Porin.pdf