Overview

Product Name

CD19 antibody

Product Number

IR253-901

Gene Description

CD19 molecule

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Recommended

Applications Dilutions

Western Blot 1:500 – 1:1000

Immunofluorescence 1:300 – 1:400

Immunohistochemistry (Paraffin) 1:300 – 1:500

Flow Cytometry 1:50 – 1:150

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR253-901 anti-CD19 antibody WB image

IR253-901 anti-CD19 antibody FACS image

All lanes : Anti-CD19 antibody at 1/800 dilution

Lysates/proteins at 60 μg per lane.

This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR253-901 overnight at 4°C.

Product：CD19 antibody

Catalog：IR253-901

Cell : Raji

FITC labelled secondary antibody.

Unlabelled sample (black) was used as a control.

Data from : National Taiwan Ocean University

IR253-901 anti-CD19 antibody ICC/IF image

IR253-901 anti-CD19 antibody IHC image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR253-901 anti- CD19 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD19 with IR253-901 at 1/400.