IR229-871 Cytokeratin 8 / CK8 antibody
Overview |
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Product Name |
Cytokeratin 8 antibody |
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Product Number |
IR229-871 |
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Gene Description |
keratin 8, type II |
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Clonality |
Polyclonal |
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Host |
Rabbit |
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Species Reactivity |
Human, Mouse |
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Recommended Applications Dilutions |
Western Blot 1:2000 – 1:3000 Immunofluorescence 1:500 – 1:800 Immunohistochemistry (Paraffin) 1:400 – 1:600 |
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Storage Buffer |
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
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Concentration |
1.51 mg/ml |
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Purity |
Affinity column purified |
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Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
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Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
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IR229-871 anti-Cytokeratin 8 antibody WB image |
IR229-871 anti-Cytokeratin 8 antibody IHC image |
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Anti-Cytokeratin 8 antibody - at 1/2000 dilution Lysates at 35 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR229-871 overnight at 4°C. |
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 8 with IR229-871 at 1/400. |
IR229-871 anti-Cytokeratin 8 antibody ICC/IF image |
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Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR229-871 anti- Cytokeratin 8 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain. |