Overview

Product Name

Cytokeratin 8 antibody

Product Number

IR229-871

Gene Description

keratin 8, type II

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse

Recommended

Applications Dilutions

Western Blot 1:2000 – 1:3000

Immunofluorescence 1:500 – 1:800

Immunohistochemistry (Paraffin) 1:400 – 1:600

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR229-871 anti-Cytokeratin 8 antibody WB image

IR229-871 anti-Cytokeratin 8 antibody IHC image

Anti-Cytokeratin 8 antibody - at 1/2000 dilution

Lysates at 35 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked an hour before being incubated with IR229-871 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 8 with IR229-871 at 1/400.

IR229-871 anti-Cytokeratin 8 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR229-871 anti- Cytokeratin 8 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.