Home
1
Products
2
IR30-79 CD133 antibody3
Home Products Primary antibody IR30-79 CD133 antibody
IR30-79

IR30-79 CD133 antibody

Overview

Product Name

CD133 / Prominin-1 antibody (Intracellular domain)

Product Number

IR30-79

Gene Description

prominin 1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:500 - 1:1000

Immunofluorescence 1:300 - 1:500

Immunohistochemistry (Paraffin) 1:200 - 1:400

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.51 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

690a239dc4590f8acf98d029e6dbb920.jpg

IR30-79 anti- CD133 / Prominin-1 antibody WB image

All lanes : Anti-CD133 antibody at 1/700 dilution

Lysates/proteins at 60 μg per lane

 

This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with IR30-79 overnight at 4°C.

Image

8dba2d61fcff510b54dbc1e8251c418c.png

IR30-79 anti- CD133 / Prominin-1 antibody ICC/IF image

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR30-79 anti- CD133 / Prominin-1 antibody (red) at 1:400 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Image

4b6c321c56f65661f3559b7943641842.jpg

IR30-79 anti- CD133 / Prominin-1 antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling CD133 / Prominin-1 with IR30-79 at 1/200.

1439590