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IR119-300 Cyclin E1 / CCNE1 antibody3
Home Products Primary antibody IR119-300 Cyclin E1 / CCNE1 antibody
IR119-300

IR119-300 Cyclin E1 / CCNE1 antibody

Overview

Product Name

Cyclin E1 antibody

Product Number

IR119-300

Gene Description

cyclin E1

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Recommended

Applications Dilutions

Western Blot 1:500-1:1000

Immunofluorescence 1:200 – 1:300

Immunohistochemistry (Paraffin) 1:50 – 1:100

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.63 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR119-300 anti-Cyclin E1 antibody WB image

IR119-300 anti-Cyclin E1 antibody ICC/IF image

IR119-300 anti-Cyclin E1 antibody IHC image

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All lanes : Anti-Cyclin E1 antibody at 1/1000 dilution

Lysates/proteins at 40 μg per lane

This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR119-300 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with IR119-300 anti-Cyclin E1 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded mouse testis labeling IL-6 with IR119-300 at 1/50.

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