IR102-426 Survivin / BIRC5 antibody
Overview |
||
Product Name |
Survivin / BIRC5 antibody |
|
Product Number |
IR102-426 |
|
Gene Description |
baculoviral IAP repeat containing 5 |
|
Clonality |
Polyclonal |
|
Host |
Rabbit |
|
Species Reactivity |
Human, Mouse, Rat |
|
Recommended Applications Dilutions |
Western Blot 1:2000-1:3000 Immunofluorescence 1:400 – 1:800 Immunohistochemistry (Paraffin) 1:100 – 1:300 |
|
Storage Buffer |
100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|
Concentration |
1.42 mg/ml |
|
Purity |
Affinity column purified |
|
Storage |
Store at +4°C for short term storage. Long time storage is recommended at -20°C |
|
Notes |
Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user. |
|
IR102-426 anti-Survivin / BIRC5 antibody WB image |
IR102-426 anti-Survivin / BIRC5 antibody ICC/IF image |
IR102-426 anti-Survivin / BIRC5 antibody IHC image |
|
|
|
Anti- Survivin / BIRC5 antibody at 1/2000 dilution Lysates at 40 μg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR102-426 overnight at 4°C. |
Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. anti- Survivin / BIRC5 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain. |
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Survivin / BIRC5 with IR102-426 at 1/150. |