Overview

Product Name

Survivin / BIRC5 antibody

Product Number

IR102-426

Gene Description

baculoviral IAP repeat containing 5

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:2000-1:3000

Immunofluorescence 1:400 – 1:800

Immunohistochemistry (Paraffin) 1:100 – 1:300

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

1.42 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR102-426 anti-Survivin / BIRC5 antibody WB image

IR102-426 anti-Survivin / BIRC5 antibody ICC/IF image

IR102-426 anti-Survivin / BIRC5 antibody IHC image

Anti- Survivin / BIRC5 antibody at 1/2000 dilution

Lysates at 40 μg per lane. This blot was produced using a 15% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR102-426 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. anti- Survivin / BIRC5 antibody (red) at 1:300 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Survivin / BIRC5 with IR102-426 at 1/150.