Overview

Product Name

EpCAM antibody (Extracellular domain)

Product Number

IR218-3

Gene Description

epithelial cell adhesion molecule

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human

Recommended

Applications Dilutions

Western Blot 1:1000 – 1:2000

Immunofluorescence 1:200 – 1:400

Immunohistochemistry (Paraffin) 1:100 – 1:150

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.54 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Image

IR218-3 anti-EpCAM antibody WB image

All lanes : Anti-EpCAM antibody - at 1/1000 dilution

Lysates/proteins at 40 μg per lane

This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane ws then blocked for an hour before being incubated with IR218-3 overnight at 4°C.

Customer feedback Image : R218-3 anti-EpCAM antibody ICC/IF image

Cell : MCF-7   (DAPI : 1-300, Phalloidin : 1-300)

EpCAM, (IR218-3) : 1-200

Anti-rabbit 594 : 1-500

NikonEpi-fluorescence (A-ken) 60x

Data from : National Yang-Ming University

IR218-3 anti- EpCAM antibody IHC image

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling EpCAM with IR218-3 at 1/200.

IR218-3 anti- EpCAM antibody FACS image

Product：EpCAM antibody

Catalog：IR218-3

Cell : MCF-7

Data from :  National Chiao-Tung University