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IR130-525 BiP / GRP78 antibody3
Home Products Primary antibody IR130-525 BiP / GRP78 antibody
IR130-525

IR130-525 BiP / GRP78 antibody

Overview

Product Name

Bip / GRP78 antibody

Product Number

IR130-525

Gene Description

heat shock protein 5

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Recommended

Applications Dilutions

Western Blot 1:2000-1:5000

Immunofluorescence 1:400 – 1:900

Immunohistochemistry (Paraffin) 1:100 – 1:300

Storage Buffer

100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative

Concentration

0.34 mg/ml

Purity

Affinity column purified

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C

Notes

Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

IR130-525 anti-BiP / GRP78 antibody WB image

IR130-525 anti-BiP / GRP78 antibody ICC/IF image

IR130-525 anti-BiP / GRP78 antibody IHC image

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Anti-BiP / GRP78 antibody at 1/3000 dilution

Lysate at 40 μg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with IR130-525 overnight at 4°C.

Cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. IR130-525 anti- Bip/ GRP78 antibody (red) at 1:500 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Bip/ GRP78 antibody with IR130-525 at 1/200.

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